BME Seminar: Dustin Tran
Monday, May 2nd, 2022, 12:00 p.m.
Dustin Tran
BME Ph.D. Candidate
Peng Lab
University of Arizona
"Light-Sheet Fluorescence Anisotropy Imaging using a Low-Cost, Polarization-Sensitive CMOS Camera"
Keating 103
Live Zoom | Passcode: BearDown
(Instructor permission required for enrolled students to attend via Zoom)
Hosts: Dr. Beth Hutchinson and Dr. Russ Witte
Persons with a disability may request a reasonable accommodation by contacting the Disability Resource Center at 621-3268 (V/TTY).
ABSTRACT: Fluorescence anisotropy imaging is a commonly used technique to study biomolecular dynamics. It utilizes the polarization properties of fluorescence emissions that occur from changes in the structure and orientation of fluorophores caused by angular displacement of the biomolecules. This can be an important tool to observe the structural organization and dynamics of protein binding for live cell studies. However, traditional fluorescence anisotropy imaging systems require the use of either multiple detectors or exposures to measure the extent of polarization in the emitted light. We present a low-cost method for performing single-exposure fluorescence anisotropy imaging using a polarization-sensitive camera. Our microscope setups utilize a 5.0 MP polarization-sensitive monochromatic CMOS sensor with an integrated wire grid polarizer array capable of performing simultaneous measurements of different polarization channels with a single detector. The setup is modular and, when combined with a scanning light-sheet microscope (LSM), can achieve high-speed, 3D functional imaging at sub-micron resolution. This capability enables functional observations of key cellular activities, such as actin cytoskeleton dynamics during cell migration.