When
Monday, April 7, 2025 at 12:00 p.m.
Brent Weyers
Senior Biosystems Applications Manager
Nikon Instruments Inc.
"Optical Sectioning in Biological Imaging: Single- vs. Multi-Photon Microscopy"
Keating 103
Zoom link | Password: BearDown
Hosts: Alex McGhee and Swarna Ganesh
(Instructor permission required for enrolled students to attend via Zoom)
Persons with a disability may request a reasonable accommodation by contacting the Disability Resource Center at 621-3268 (V/TTY).
Abstract: This seminar will provide an overview of the physics and applications of single- and multi-photon microscopy, explaining how both modalities achieve optical sectioning. Topics will include optical scattering and absorption, tissue optical clearing, and a high-level discussion of fluorescence physics for each technique. The second part of the seminar will focus on Nikon’s multi-photon microscope systems, including key hardware components such as lasers and detectors. Representative datasets will be showcased, demonstrating imaging possibilities across live organisms and fixed samples.
Bio: Brent Weyers is part of Nikon's microscopy training team for the biological solutions and healthcare division. He earned his PhD from the University of California, Davis, where he developed and applied a fiber-optic-based Fluorescence Lifetime Imaging (FLIm) system for real-time surgical guidance in head and neck oncology procedures. As a postdoctoral researcher, he further advanced FLIm applications by integrating machine learning models to enhance sensitivity and specificity in neurological surgical oncology.
At Nikon, Weyers focuses on internal employee microscopy training, developing online customer eLearning courses, and providing optics education through seminars and hands-on training.