David W. Galbraith
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Professor of Plant Sciences
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Professor of Biomedical Engineering
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Professor, BIO5 Institute
Brief statement of professional interests.
I have a long track record of devising novel methods and platforms for the analysis of the different cell types found within complex eukaryotic organisms. I further have a long history of collaborative research activity, research publication, and a 30+ year record of continuous federal funding, from single investigator to complex multi-investigator, multi-institution projects. I provide unique molecular expertise for the study of gene expression in normal development and in disease, focused on biological and biomedical instrumentation. I am interested in all kingdoms of life, including plants and animals, and recently have developed interests in devising methods and instruments for detection of pathogens.
Degrees
- Ph.D. Plant Biochemistry
- Cambridge University, Cambridge, England
- Membranes of soybean callus
- M.A. Plant Biochemistry
- Cambridge University, Cambridge, England
- B.A. Natural Sciences Tripos (Biochemistry)
- Cambridge University, Cambridge, England
Work Experience
- Henan University, School of Life Sciences (2018 - Ongoing)
- University of Arizona, Tucson, Arizona (1989 - Ongoing)
- University of Nebraska-Lincoln, Lincoln, Nebraska (1984 - 1988)
- University of Nebraska-Lincoln, Lincoln, Nebraska (1978 - 1984)
- Stanford University, Palo Alto, California (1976 - 1978)
Courses
Feed & Clothe 9-Billion People
PLS 195A (Fall 2022)
PLS 195A (Fall 2021)
PLS 195A (Fall 2020)
PLS 195A (Fall 2019)
PLS 195A (Fall 2018)
PLS 195A (Fall 2017)
PLS 195A (Fall 2016)
Special Topics in Science
HNRS 195I (Spring 2019)
HNRS 195I (Spring 2018)
HNRS 195I (Fall 2016)
Senior Capstone
PLS 498 (Spring 2022)
PLS 498 (Spring 2021)
Mthd Cell Bio+Genomics
GENE 539 (Fall 2022)
GENE 539 (Fall 2021)
GENE 539 (Fall 2020)
GENE 539 (Fall 2018)
GENE 539 (Fall 2016)
MCB 539 (Fall 2022)
MCB 539 (Fall 2021)
MCB 539 (Fall 2020)
MCB 539 (Fall 2018)
PCOL 539 (Fall 2022)
PCOL 539 (Fall 2020)
PLS 539 (Fall 2022)
PLS 539 (Fall 2021)
PLS 539 (Fall 2020)
PLS 539 (Fall 2018)
PLS 539 (Fall 2016)
PSIO 539 (Fall 2021)
PSIO 539 (Fall 2020)
PSIO 539 (Fall 2016)
Rsrch Meth Biomed Engr
BME 597G (Fall 2019)
BME 597G (Fall 2017)
Selected Publications
Chapters
- Lambert, G. M., & Galbraith, D. W. (2020). Advances in the Flow Cytometric Characterization of Plant Cells and Tissues. In Flow Cytometry Applications in Cell Culture. CRC Press. doi:10.1201/9781003067467-18
- Galbraith, D. W. (2009). The Wonderland of Global Expression Profiling. In Molecular Genetic Approaches to Maize Improvement. Springer, Berlin, Heidelberg. doi:10.1007/978-3-540-68922-5_18
- Galbraith, D. W., & Bartos, J. (2005). Flow Cytometry and Cell Sorting in Plant Biotechnology. In Flow Cytometry for Biotechnology. Oxford University Press. doi:10.1093/OSO/9780195183146.003.0021
- Galbraith, D. W., & Lucretti, S. (2000). Large Particle Sorting. In Flow Cytometry and Cell Sorting. Springer, Berlin, Heidelberg. doi:10.1007/978-3-662-04129-1_25
- Galbraith, D. W. (1991). Fluorescence-activated analysis and sorting of protoplasts and somatic hybrids. In Plant Tissue Culture Manual.. Springer, Dordrecht. doi:10.1007/978-94-009-0103-2_38
- Galbraith, D. W. (1984). CHAPTER 50 – Selection of Somatic Hybrid Cells by Fluorescence-Activated Cell Sorting. In Cell Culture and Somatic Cell Genetics of Plants. doi:10.1016/B978-0-12-715001-7.50057-3
- Galbraith, D. W. (1984). CHAPTER 83 – Flow Cytometric Analysis of the Cell Cycle. In Cell Culture and Somatic Cell Genetics of Plants. doi:10.1016/B978-0-12-715001-7.50090-1
- Galbraith, D. W. (1983). RAPID FLOW CYTOMETRIC ANALYSIS OF NUCLEAR DNA LEVELS IN HIGHER PLANTS. In Advances in Gene Technology: Molecular Genetics of Plants and Animals. doi:10.1016/B978-0-12-221480-6.50046-3
Journals/Publications
- Antoniadi, I., Skalický, V., Sun, G., Ma, W., Galbraith, D. W., Novák, O., & Ljung, K. (2021). Fluorescence activated cell sorting-A selective tool for plant cell isolation and analysis. Cytometry. Part A : the journal of the International Society for Analytical Cytology.
- Galbraith, D. W. (2021). From mouse to mouse-ear cress: nanomaterials as delivery carriers in plant biotechnology.. Exploration, 1(1), 9-20. doi:https://doi.org/10.1002/EXP.20210002
- Galbraith, D. W. (2021). Shapiro's Laws Revisited: Conventional and Unconventional Cytometry at CYTO2020.. Cytometry. Part A : the journal of the International Society for Analytical Cytology, 99(2), 129-132. doi:10.1002/cyto.a.24228
- Galbraith, D. W. (2021). Shapiro's Laws Revisited: Conventional and Unconventional Cytometry at CYTO2020. Cytometry. Part A : the journal of the International Society for Analytical Cytology, 99(2), 129-132.
- Galbraith, D. W. (2021). Validation of crowd-sourced plant genome size measurements. Cytometry. Part A : the journal of the International Society for Analytical Cytology.
- Galbraith, D. W., & Sun, G. (2021). Flow Cytometry and Sorting in Arabidopsis. Methods in molecular biology (Clifton, N.J.), 2200, 255-294.
- Sliwinska, E., Loureiro, J., Leitch, I. J., Šmarda, P., Bainard, J., Bureš, P., Chumová, Z., Horová, L., Koutecký, P., Lučanová, M., Trávníček, P., & Galbraith, D. W. (2021). Application-based guidelines for best practices in plant flow cytometry. Cytometry. Part A : the journal of the International Society for Analytical Cytology.
- Sun, G., & Galbraith, D. W. (2021). Flow Cytometry and Sorting in Arabidopsis.. Methods in molecular biology (Clifton, N.J.), 2200, 255-294. doi:10.1007/978-1-0716-0880-7_12
- Čertnerová, D., & Galbraith, D. W. (2021). Best practices in the flow cytometry of microalgae. Cytometry. Part A : the journal of the International Society for Analytical Cytology, 99(4), 359-364.
- Galbraith, D. W., Shi, Z., Halaly-Basha, T., Zheng, C., Wang, C., Ophir, R., Pang, X., & Or, E. (2020). Identification of post-ethylene events in the signaling cascade induced by dormancy release stimuli.. Plant Journal, 104(5), 1251-1268. doi:DOI:10.1111/tpj.14997.
- Galbraith, D. W., Yan, Q., Bai, S., Li, W., Zhou, Y., Yang, Z., Sun, G., & Wang, B. (2020). Transcriptome analysis reveals key genes involved in the regulation of nicotine biosynthesis at early time points after topping in tobacco (Nicotiana tabacum L.).. B.M.C. Plant Biology, 20, Art. No. 30. doi:https://doi.org/10.1186/s12870-020-2241-9
- Munawar, N., Mahmood, T., & Galbraith, D. W. (2020). Chemical control of rodents and its impact on rodent infestations during subsequent cropping season. International Journal of Pest Management, 1-10. doi:10.1080/09670874.2020.1861362
- Qin, Y., Bai, S., Li, W., Sun, T., Galbraith, D. W., Yang, Z., Zhou, Y., Sun, G., & Wang, B. (2020). Transcriptome analysis reveals key genes involved in the regulation of nicotine biosynthesis at early time points after topping in tobacco (Nicotiana tabacum L.). BMC plant biology, 20(1), 30.
- Shahid, A. A., Rao, A. Q., Latif, A., Jabbar, B., Iqbal, A., Husnain, T., Gul, A., Galbraith, D. W., Ali, M. A., & Ahmed, M. (2020). Structure-based prediction of protein–protein interactions between GhWlim5 Domain1 and GhACTIN-1 proteins: a practical evidence with improved fibre strength. Journal of Plant Biochemistry and Biotechnology, 1-14. doi:10.1007/s13562-020-00603-7
- Shi, Z., Halaly-Basha, T., Zheng, C., Sharabi-Schwager, M., Wang, C., Galbraith, D. W., Ophir, R., Pang, X., & Or, E. (2020). Identification of potential post-ethylene events in the signaling cascade induced by stimuli of bud dormancy release in grapevine. The Plant journal : for cell and molecular biology, 104(5), 1251-1268.
Proceedings Publications
- Rodriguez, J. J., Xia, B., Sankaranarayanan, S., Rodriguez, J. J., Murthi, S., & Galbraith, D. W. (2002). Improved data acquisition system for digital flow cytometry. In 2002 IEEE International Symposium on Circuits and Systems. Proceedings (Cat. No.02CH37353), 1, 669-672.
- Rodriguez, J. J., Yopp, T. A., Rodriguez, J. J., Lambert, G. M., Godavarti, M., & Galbraith, D. W. (1995). Neural network analysis of digital flow cytometric data. In Proceedings of ICNN'95 - International Conference on Neural Networks, 5, 2211-2216.
Presentations
- Galbraith, D. W. (2021, December). Cytotaxonomy. UCD Conway Flow Cytometry eWinter School. Virtual (Zoom): University College Dublin -- Conway.
- Galbraith, D. W. (2021, January). Advanced technologies for investigating the complex regulatory mechanisms of eukaryotes at the level of single cells. Academic Forum of Interdisciplinary Biology, & Inaugural Ceremony of Exploration. Kaifeng, China (via Zoom): Henan University, Chinese Association of Nanobiology, Wiley, and Exploration.
- Galbraith, D. W. (2021, June). Biological Applications of Cytometry: Flow Cytometry and Sorting for monitoring Cell Fate in Maize. Annual Meeting of the Society for In Vitro Biology. On-line (Zoom): Society for In Vitro Biology.
- Galbraith, D. W. (2021, June). Grow with the Flow! -- Creative Changes in Advanced Flow Cytometry for the 21st Century. Society for In Vitro Biology Meeting 2021. On-line (Zoom): Society for In Vitro Biology.
- Galbraith, D. W. (2021, November). Non-mammalian cytometry. Annual Australasian Cytometry Society Conference 2021. On-line (Zoom): Australasian Cytometry Society.
- Galbraith, D. W. (2019, February). Analyzing Gene Expression, Agnostically, at the Level of Single Cells and Nuclei . Gordon Research Conference on Quantitative Genetics and Genomics. Il Ciocco, Italy.: Gordon Research Conferences.
- Galbraith, D. W. (2019, February). Preparation of vaccines in plants. VacciniAMO Conference. University of Modena, Italy.: University of Modena.
- Galbraith, D. W. (2019, January). Gene Expression Analysis. XXVIIth Plant and Animal Genome Meeting. San Diego: PAG.
- Galbraith, D. W. (2019, June). Fluorescent Proteins in Flow and Image Cytometry. 42nd Annual Course in Flow Cytometry University of Wisconsin. Madison: University of Wisconsin.
- Galbraith, D. W. (2019, March). There is no Planet B. TEDxUofA: Abberance. University of Arizona: TEDxUofA.
- Galbraith, D. W. (2019, November). Future Flow: Where will Cytometry take Us?. First International Workshop on Exotic Flow Cytometry: Checking Small Things... Better! ExoFlowMetry. ENEA Casaccia, Rome, Italy (Keynote Speaker).. Rome, Italy: ENEA Casaccia.
- Galbraith, D. W., & Villalobos-Menuey, L. (2019, June). Workshop: Flow cytometry of plants: a practical introduction to angiosperm genome size measurement . Botany 2019 Conference in Tucson, Arizona. Tucson: Botanical Society of America.
- Galbraith, D. W., Sun, G., Song, C., Xia, M., Li, J., Guo, G., & Fang, S. (2019, June). Agnostic clustering of RNA-Seq transcripts from single sorted nuclei identifies novel cell types within maize organs. CYTO2019, Vancouver, Canada.. Vancouver: International Society for Advancement of Cytometry.
- Galbraith, D. W. (2018, May). Cell-type Discovery within Complex Tissues and Organs using Flow Cytometry and Sorting. Seminar: Max Planck Institute of Molecular Plant Physiology. Golm: Max Planck Institute.
- Galbraith, D. W. (2018, Spring). Advances in the cytometry of single cells and organelles . 2018 Korea University-University of Arizona Joint International Symposium in Biomedical Engineering. Bio5 Institute, University of Arizona, Tucson: Korea University and University of Arizona.
Others
- Galbraith, D. W. (2013, July). Participate in Gates Foundation Workshop on African Agriculture. Lake Naivasha. Kenya.
- Galbraith, D. W. (2010, Fall). SPLS website.